Translational_Unit

Part:BBa_K993000:Design

Designed by: ABDÜLKADİR KARADAĞ   Group: iGEM13_AUC_TURKEY   (2013-06-14)


RFP Generator with ROSE thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 748
    Illegal AgeI site found at 860
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.


Source

Bradyrhizobium japonicum USDA 110 and Registry

References